Precision through imaging
Our approach combines image-guidance, precision, adaptability, and pioneering spirit to drive innovative advancements in our research methodologies.
Image Guidance
Until recently, interventions in large animals relied on invasive open surgeries. In contrast, image-guided and minimally invasive procedures have gained significant momentum in human medicine. This shift opens up a vast array of opportunities, allowing for the transition from open surgeries to minimally invasive interventions in preclinical settings. This advancement not only accelerates the adoption of precision medicine paradigms but also paves the way for aligning animal research more closely with the rapidly evolving clinical landscape.
Our objective is to bridge the gap and align with clinical practices by introducing image-guided animal procedures. Our approach closely mirrors the clinical revolution, offering a foundation for accelerated innovation. A paradigm shift is underway, emphasizing minimally invasive catheter placement or entirely non-invasive, focal induction of bodily changes. Advanced imaging enables these procedures to be conducted in a spatially rational manner, often providing nearly real-time feedback on the progress of the intervention. Of particular significance is the compelling capability for labeling and imaging the delivery of therapeutic agents to the brain and beyond.
Magnetic resonance imaging
Harnessing the power of magnetic resonance imaging (MRI), we employ a sophisticated and non-invasive approach to meticulously evaluate disease progression and treatment efficacy. In particular we employ interventional MRI as cutting-edge technology enabling us to delve into the intricacies of dynamic process during disease model induction, drug administration and therapeutic responses, providing valuable insights into the effectiveness of treatments. By leveraging MRI, we not only track the evolution of diseases with precision but also contribute to the development of targeted and efficient therapeutic strategies. Our commitment to utilizing advanced imaging techniques ensures a comprehensive understanding of the dynamic interplay between diseases and interventions, paving the way for more informed and successful medical outcomes.
Computed tomography (CT)
CT is a versatile imaging tool that finds valuable applications in our portfolio for assessment of large animal models of ischemic and hemorrhagic stroke. Here are examples of how CT is being utilized: verification of telemetry device positioning, skeletal structure analysis, hematoma evaluation, in the context of hemorrhagic stroke models. Vascular Imaging such as CT angiography or perfusion imaging. By incorporating CT into the portfolio of imaging modalities, these applications enhance the precision and depth of data collection, contributing to a more comprehensive understanding of stroke models and facilitating the development of novel therapeutic strategies.
Telemetry
The utilization of telemetry techniques in large animal studies focused on central nervous system diseases offers a multitude of advantages. This sophisticated approach allows for real-time, continuous, objective monitoring of physiological parameters, providing a dynamic and comprehensive understanding of the intricate complexities involved. Telemetry not only minimizes the impact on animal welfare by eliminating the need for invasive procedures but also enhances the precision and reliability of data collection.
Moreover, telemetry facilitates the exploration of circadian rhythms and the influence of various factors on disease manifestations, offering valuable insights that may be challenging to capture through traditional intermittent monitoring methods. In essence, the incorporation of telemetry techniques in large animal studies represents a leap forward in enhancing both the ethical considerations and scientific rigor of research focused on central nervous system diseases.
Histology
Our research methodology includes the capability to conduct histopathological assays, providing a comprehensive and detailed examination of tissue samples. This technique allows for a meticulous analysis of cellular and tissue structures, offering a closer look at the intricacies of pathological changes. Through histopathology, we enhance our understanding of disease processes, assess treatment impacts, and contribute to the refinement of therapeutic strategies. This powerful tool in our research arsenal deepens our insights into biological mechanisms and also strengthens the scientific foundation for advancements in preclinical studies and the development of targeted interventions.
Flow cytometry
In our research endeavors, we leverage the advanced techniques for isolation of critical cell populations from tissues for flow cytometry analysis to gain a comprehensive understanding of cellular composition and dynamics. This method allows us to unravel the complexities of immune responses, assess cellular functions, and delve into the intricacies of disease processes.
Most frequent asked questions
Some questions appear very often, so we decided to dispel doubts.
Why should we use large animal models?
Large animal models are increasingly used in neurological disease modeling and are particularly appreciated in preclinical neuroendovascular research. These animals offer numerous advantages, such as larger vessel sizes compared to rodents, enabling the use of catheters used in patients, as well as clinical magnetic resonance (MRI), computed tomography (CT), and positron emission tomography (PET) scanners. The gyrencephalic brain anatomy of large experimental animals and the human-like gray to white matter ratio make large animal models more closely reflect the clinical situation. Despite slight anatomical differences in some species of large animals, the cerebral blood supply and cerebrovascular architecture are very similar to those of humans. Large animal models are also excellent for long-term research and monitoring therapeutic progress. The use of species with gyrencephalic brain anatomy may facilitate direct translation of the results of research experiments into the clinic. Furthermore, the utilization of large animals, particularly pigs, in research offers a distinct advantage due to their close clinical relevance. Pigs have the capacity to develop conditions like obesity and metabolic syndrome, which are highly prevalent co-morbidities in humans. By modeling these conditions in swine, either independently or in conjunction with other diseases like stroke, it is possible to achieve a more accurate representation of the intricate disease complexities. This approach enhances the ability to discern and develop innovative and pertinent treatment strategies.
Why are you promoting intra-arterial administration of drugs?
The rapid progress in the field of biotechnology achieved over the last few years has led to the development of a wide range of drugs based on antibodies, nanobodies, and various peptides. Due to the large size of these molecules and the resulting impenetrability of the blood-brain barrier, they did not bring the intended therapeutic effects in neurological diseases. The use of endovascular techniques for direct infusion of therapeutic agents into cerebral vessels allows achieving high drug concentrations in the target tissue while minimizing systemic toxicity. The advantages of intra-arterial administration of therapeutic agents under imaging guidance, apart from low invasiveness, include high repeatability, enabling a systemic biological effect and wide distribution of the therapeutic agent in the brain, as well as the ability to preview the tissue response to the therapy.
What standard services do you provide?
Safety, feasibility and efficacy studies performed by Ti-com for neurological diseases typically feature project planning and design, bioethics committee application, broad spectrum of neurosurgical interventions, clinical observations, blood testing, MR imaging, CT imaging, body weights, longitudinal neurological scoring, survival, tissue/blood collection, ultrasonography, electrocardiography (ECG), electroencephalography (EEG) histological assessment and more. We are ready to tailor our service portfolio according to the specific needs of each individual project.